Molecular 'trick' enables 8x increase in efficiency of CRISPR-Cas9  for precise genetic modifications

03/25/2015 - 00:00

GIF above is from an MIT video "Genome Editing with CRISPR-Cas9"

CRISPR-Cas9 is a powerful new tool for editing the genome. For researchers around the world, the CRISPR-Cas9 technique is an exciting innovation because it is faster and cheaper than previous methods. Now, using a molecular trick, Dr. Van Trung Chu and Professor Klaus Rajewsky of the Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch and Dr. Ralf Kühn, MDC and Berlin Institute of Health (BIH), have found a solution to considerably increase the efficiency of precise genetic modifications by up to eightfold.

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Ref: Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.  Nature Biotechnology (2015) doi:10.1038/nbt.3198